Transfer as a reciprocal process: How to foster receptivity to results of transdisciplinary research

Transdisciplinary research (TDR) seeks to address real-world problems and aims to be socially transformative. This normative objective extends beyond particular TDR projects, as real-world problems are embedded in concrete contexts but, at the same time, are also related to wider societal challenges that are not restricted to one context. Therefore, TDR generally entails transfer of knowledge and results to other contexts. However, the TDR discourse has mainly treated transfer efforts from the perspective of scientific generalization, translation and packaging of knowledge. Within this understanding of transfer, little attention has been paid to interplay between contexts and the role of new contexts themselves. This article is based on qualitative explorative research on four TDR projects. Its results were iteratively derived through project analysis, reflection on insights from the literature and discussions with TDR experts. We propose that transfer is a complex reciprocal process in which different types of knowledge are provided and transferred to other contexts, where knowledge is adapted, enriched and modified. In addition to project researchers, actors in other (pick-up) contexts also play an important role for successful transfer and appropriation of TDR results. Generating transfer potential within the duration of a project depends on being aware of potential pick-up contexts. To address the interdependent aspects of transfer (results, mediation, and appropriation in other contexts), we present a comprehensive model outlining TDR transfer processes. To support projects seeking to raise their transfer potential in a more conscious manner, we also formulate three overarching recommendations: 1) process results for transfer adequately, 2) identify and support intermediaries and, 3) increase awareness of and address other contexts. Considering these recommendations while also being aware of their interdependence may increase potential for transfer of knowledge and results to other contexts. Our conceptual understanding acknowledges the complexity and non-linearity of endeavors to take advantage of case-specifically gained knowledge and results in other contexts or at other scales

Societal effects of transdisciplinary sustainability research—How can they be strengthened during the research process?

Transdisciplinary sustainability research aims to mitigate or to solve complex societal problems and advance the production of scientific knowledge. Reflexive approaches to transdisciplinary research processes are outlined to systematically strengthen the potential for societal effectiveness. So far, it is rare to find empirically based analyses of the links between the quality of the research process and the methods applied on the one hand and the effects achieved on the other. This paper thus addresses the issue of heightening the societal effects of transdisciplinary sustainability research. The objective is to explore ways of consciously promoting societal effectiveness in transdisciplinary research. We argue that these possibilities evolve at the intersection between the general project framework and an adaptive shaping of transdisciplinary research processes. A reflexive approach of this kind proactively considers the dynamics of interests and concerns, roles and responsibilities, the collaboration culture within a project, and the connectivity to the context of action addressed. Its deployment presupposes an appreciation of the basic conditions, i.e. the historical development of the respective problem, the heterogeneity of actors involved, the general environment and, finally, the funding conditions

The Structural Analysis of the Sectors of Slovak Economy and Employment in Times of Global Change and the Subsequent Development

AbstractThe regions situated in the European environment have different primary sources, their quality and their level of utilization, which have a direct influence on their subsequent performance and productivity. The aim of this contribution is to evaluate the performance of the Slovak economy and its sectors in relation to employment in the reference period from 2000 to 2012. This analysis confirmed our assumption. From conducted analysis of position of the individual sectorsis is evident that the industries of primary and secondary sector lost its intensity and recorded deceleration of the performance and productivity. The tertiary sector in Slovakia in the reference period contributes utmost to formation of gross domestic product and employment creation. Productivity growth of the individual sectors of Slovak economy and their competitiveness will be directly proportionally related to the ability of the enterprises to respond successfully to new challenges in the field of innovations and the optimal use of production resources

Wirkung und gesellschaftliche Wirksamkeit

Wie kann die gesellschaftliche Wirkung von transdisziplinären Forschungsprojekten erfasst und bewertet werden? Diese Frage steht im Fokus des vorliegenden Textes. Es wird deutlich, dass es bisher keine einheitlichen Begrifflichkeiten und Indikatoren gibt, um die gesellschaftliche Wirksamkeit von transdisziplinären Projekten zu messen. Qualitative Ansätze wie Fallstudien und Wirkungsnarrative werden häufig eingesetzt, um der gesellschaftlichen Wirksamkeit auf die Spur zu kommen. Auch quantitative Kenngrößen werden gesucht, die dem transdisziplinären Forschungsmodus gerecht werden. Eine systematische Erfassung und Darstellung von Wirkungen transdisziplinärer Projekte ist bisher forschungspolitisch nicht mit entsprechenden Programmen und Finanzierungsmöglichkeiten unterlegt. Es besteht Handlungsbedarf in der Anpassung der universitären Lehre, um die Realisierung wirkungsvoller transdisziplinärer Forschung zu ermöglichen. (Herausgeber)How can the social impact of transdisciplinary research projects be recorded and evaluated? This question is the focus of the present text. It becomes clear that so far there are no standardized terminologies and indicators to measure the societal effectiveness of transdisciplinary projects. Qualitative approaches such as case studies and impact narratives are often used to track social effectiveness. Quantitative indicators are also sought that do justice to the transdisciplinary research mode. Systematic recording and presentation of the effects of transdisciplinary projects has not yet been backed up by appropriate programs and funding opportunities in terms of research policy. There is a need for action in the adaptation of university teaching to enable the realization of effective transdisciplinary research. (Editor

Social logistics: Rethinking public transport as a regional distributor of goods in rural areas. Chances for the long-term establishment of a sustainable regional logistics approach

This paper presents initial findings on the establishment of a social logistics project in a peripheral rural region in Germany. As part of a real-life laboratory, two services were developed and tested with the aim of improving rural supply: the delivery of regional goods and the return of online orders in cooperation with a regional public transport provider, regional retailers and parcel services. An evaluation team provided support to the project in identifying, monitoring, and documenting the societal impact of the services and promoted impact-oriented project management. The concept of providing services of general interest through social logistics and testing them in a real-world laboratory was based on preliminary work in the region. The results indicate that this approach can promote this type of social logistics in the long term. A social logistics approach does not focus solely on economic aspects but emphasises social and environmental benefits for the region. A real-world laboratory approach enables an environment that promotes open exchange, involvement of different stakeholders and joint problem solving in a reflective, iterative process. This methodological approach enabled the continuation and out-scaling of the service’s core offerings immediately after ending the test phase and is seen as an important prerequisite for establishing a long-term perspective

Utilization of Benchtop Next Generation Sequencing Platforms Ion Torrent PGM and MiSeq in Noninvasive Prenatal Testing for Chromosome 21 Trisomy and Testing of Impact of In Silico and Physical Size Selection on Its Analytical Performance

OBJECTIVES: The aims of this study were to test the utility of benchtop NGS platforms for NIPT for trisomy 21 using previously published z score calculation methods and to optimize the sample preparation and data analysis with use of in silico and physical size selection methods. METHODS: Samples from 130 pregnant women were analyzed by whole genome sequencing on benchtop NGS systems Ion Torrent PGM and MiSeq. The targeted yield of 3 million raw reads on each platform was used for z score calculation. The impact of in silico and physical size selection on analytical performance of the test was studied. RESULTS: Using a z score value of 3 as the cut-off, 98.11% - 100% (104-106/106) specificity and 100% (24/24) sensitivity and 99.06% - 100% (105-106/106) specificity and 100% (24/24) sensitivity were observed for Ion Torrent PGM and MiSeq, respectively. After in silico based size selection both platforms reached 100% specificity and sensitivity. Following the physical size selection z scores of tested trisomic samples increased significantly-p = 0.0141 and p = 0.025 for Ion Torrent PGM and MiSeq, respectively. CONCLUSIONS: Noninvasive prenatal testing for chromosome 21 trisomy with the utilization of benchtop NGS systems led to results equivalent to previously published studies performed on high-to-ultrahigh throughput NGS systems. The in silico size selection led to higher specificity of the test. Physical size selection performed on isolated DNA led to significant increase in z scores. The observed results could represent a basis for increasing of cost effectiveness of the test and thus help with its penetration worldwide

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Dissociated Representations of Pleasant and Unpleasant Olfacto-Trigeminal Mixtures: An fMRI Study

How the pleasantness of chemosensory stimuli such as odorants or intranasal trigeminal compounds is processed in the human brain has been the focus of considerable recent interest. Yet, so far, only the unimodal form of this hedonic processing has been explored, and not its bimodal form during crossmodal integration of olfactory and trigeminal stimuli. The main purpose of the present study was to investigate this question. To this end, functional magnetic resonance imaging (fMRI) was used in an experiment comparing brain activation related to a pleasant and a relatively unpleasant olfacto-trigeminal mixture, and to their individual components (CO2 alone, Orange alone, Rose alone). Results revealed first common neural activity patterns in response to both mixtures in a number of regions: notably the superior temporal gyrus and the caudate nucleus. Common activations were also observed in the insula, although the pleasant mixture activated the right insula whereas the unpleasant mixture activated the left insula. However, specific activations were observed in anterior cingulate gyrus and the ventral tegmental area only during the perception of the pleasant mixture. These findings emphasized for the firs time the involvement of the latter structures in processing of pleasantness during crossmodal integration of chemosensory stimuli

Heterozygous Loss-of-Function Mutations in DLL4 Cause Adams-Oliver Syndrome.

Adams-Oliver syndrome (AOS) is a rare developmental disorder characterized by the presence of aplasia cutis congenita (ACC) of the scalp vertex and terminal limb-reduction defects. Cardiovascular anomalies are also frequently observed. Mutations in five genes have been identified as a cause for AOS prior to this report. Mutations in EOGT and DOCK6 cause autosomal-recessive AOS, whereas mutations in ARHGAP31, RBPJ, and NOTCH1 lead to autosomal-dominant AOS. Because RBPJ, NOTCH1, and EOGT are involved in NOTCH signaling, we hypothesized that mutations in other genes involved in this pathway might also be implicated in AOS pathogenesis. Using a candidate-gene-based approach, we prioritized DLL4, a critical NOTCH ligand, due to its essential role in vascular development in the context of cardiovascular features in AOS-affected individuals. Targeted resequencing of the DLL4 gene with a custom enrichment panel in 89 independent families resulted in the identification of seven mutations. A defect in DLL4 was also detected in two families via whole-exome or genome sequencing. In total, nine heterozygous mutations in DLL4 were identified, including two nonsense and seven missense variants, the latter encompassing four mutations that replace or create cysteine residues, which are most likely critical for maintaining structural integrity of the protein. Affected individuals with DLL4 mutations present with variable clinical expression with no emerging genotype-phenotype correlations. Our findings demonstrate that DLL4 mutations are an additional cause of autosomal-dominant AOS or isolated ACC and provide further evidence for a key role of NOTCH signaling in the etiology of this disorder